stc2 shrna h particles  (Santa Cruz Biotechnology)

Journal: Nature Communications

Article Title: Electrical modulation of transplanted stem cells improves functional recovery in a rodent model of stroke

doi: 10.1038/s41467-022-29017-w

Figure Lengend Snippet: a Heatmap of transcriptome changes in NPCs from electrical stimulation (NPC – unstimulated, NPC Stim – electrically stimulated; blue-downregulated, orange-upregulated). b Volcano plot demonstrating changes in genes with electrical stimulation (blue-downregulated, orange-upregulated). c Top Gene set enrichment pathways with STC2 as the leading edge. d qRT-PCR analysis indicated that STC2 in NPCs was upregulated by electrical stimulation. e ELISA study indicated that the level of STC2 protein after electrical stimulation (NPC Stim ) is much higher than that in non-stimulated NPCs (NPC). d , e Analyzed using a one-way ANOVA, followed by Tukey’s HSD post-hoc test with ** P < 0.01, **** P < 0.0001, data shown as mean ± SEM, n = 4.

Article Snippet: For STC2 gain and loss of function, hNPCs were treated with STC2 lentiviral activation particles (sc-405292-LAC) and STC2 shRNA (h) particles (sc-44127-V) respectively as per the manufacturer protocol (Santa Cruz Biotechnology Inc., Lentiviral activation particles transduction).

Techniques: Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay

Journal: Nature Communications

Article Title: Electrical modulation of transplanted stem cells improves functional recovery in a rodent model of stroke

doi: 10.1038/s41467-022-29017-w

Figure Lengend Snippet: a Vibrissae-forepaw (WP) and total neurological score (NS). There is a statistically significant difference in NPC Stim , STC2 UP , and Scramble KD+Stim groups versus all other groups starting at week 3 and onward for WP Scores and week 5 and onward for NS scores. STC2 KD and STC2 KD+Stim showed no significant behavioral recovery at any timepoint in either WP Scores or NS Scores. b Representative images of fluorescently labeled cells. Green indicates BrdU positive cells, whereas Blue indicates cell nucleus. Scale bar indicates 200 µm. c BrdU positive cells in certain areas of interest. Data are shown as mean ± SEM, n = 8 images/4 rats. d PAX6/BrdU or Nestin/BrdU positive cells in certain areas of interest. Data are shown as mean ± SEM, n = 16 images/4 rats. e Representative images of fluorescently labeled cells. Green indicates Nestin positive cells whereas Red indicates PAX6 positive cells. Blue indicates BrdU positive cells. Scale bar indicates 200 µm. a Analyzed using a Kruskal–Wallis test followed by post-hoc pairwise Mann–Whitney U test with Benjamini–Hochberg correction to control the false discovery rate at the 0.05 level. For both, * P < 0.01, ** P < 0.001, data shown as mean ± SEM, n = 10 per group. c , d Analyzed using a one-way ANOVA, followed by Tukey’s HSD post-hoc test with * P < 0.05, ** P < 0.01, and **** P < 0.0001.

Article Snippet: For STC2 gain and loss of function, hNPCs were treated with STC2 lentiviral activation particles (sc-405292-LAC) and STC2 shRNA (h) particles (sc-44127-V) respectively as per the manufacturer protocol (Santa Cruz Biotechnology Inc., Lentiviral activation particles transduction).

Techniques: Labeling, MANN-WHITNEY, Control

Journal: Nature Communications

Article Title: Electrical modulation of transplanted stem cells improves functional recovery in a rodent model of stroke

doi: 10.1038/s41467-022-29017-w

Figure Lengend Snippet: a Schematic of the brain slice view (left) and top view (right) in the skull for STC2 protein delivery via mini-osmotic pump. The cannula of the osmotic pump is implanted at 0.8 mm posterior (Y) and 1.5 mm contralateral (X) to the bregma at a depth of 3.5 mm at 1-week after stroke. b Behavioral testing. Vibrissae-forepaw (WP) behavioral testing (left) and overall neurological score (right). Based on WP, STC2 group exhibited statistically significantly greater recovery beginning at 4 weeks post-stroke compared to other groups. c Endogenous neuroblasts. Representative images of DCX + /BrdU+ cells (left) and the cell count of number co-positive cells (right) at peri-infarct region. b Analyzed using a log-transform two-way repeated measures ANOVA which revealed a statistically significant interaction between the effects of treatment group and WP Score ( F [12, 162] = 7.058, P < 0.0001); followed by Dunnett’s multiple comparisons test. Neurological scores (NS) were analyzed using a Kruskal–Wallis test ( P = 0.018), followed by Dunn’s multiple comparisons test. For both, * P < 0.05, ** P < 0.01, data shown as mean ± SEM, n = 10 per group. c Analyzed using one-way ANOVA, followed by Tukey’s HSD post-hoc test with * P < 0.05. Data shown as mean ± SEM, n = 20 images/4 rats.

Article Snippet: For STC2 gain and loss of function, hNPCs were treated with STC2 lentiviral activation particles (sc-405292-LAC) and STC2 shRNA (h) particles (sc-44127-V) respectively as per the manufacturer protocol (Santa Cruz Biotechnology Inc., Lentiviral activation particles transduction).

Techniques: Slice Preparation, Cell Counting